pcag ires gfp backbone (New England Biolabs)

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New England Biolabs pcag ires gfp backbone
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Pcag Ires Gfp Backbone, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 15375 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcag+ires+gfp+backbone/EcoRI/pmc08163098-1537-17-30
Average 99 stars, based on 15375 article reviews

pcag ires gfp backbone - by Bioz Stars, 2026-09

99/100 stars

Images

1) Product Images from "Protein Synthesis in the Developing Neocortex at Near-Atomic Resolution Reveals Ebp1-Mediated Neuronal Proteostasis at the 60S Tunnel Exit"

Article Title: Protein Synthesis in the Developing Neocortex at Near-Atomic Resolution Reveals Ebp1-Mediated Neuronal Proteostasis at the 60S Tunnel Exit

Journal: Molecular cell

doi: 10.1016/j.molcel.2020.11.037

Figure Legend Snippet: KEY RESOURCES TABLE

Techniques Used: Recombinant, Luciferase, shRNA, Transfection, Protease Inhibitor, Plasmid Preparation, Mass Spectrometry, RNA Sequencing Assay, Software, Over Expression

Figure Legend Snippet: KEY RESOURCES TABLE

Techniques Used: Recombinant, Luciferase, shRNA, Transfection, Protease Inhibitor, Plasmid Preparation, Mass Spectrometry, RNA Sequencing Assay, Software, Over Expression

Cloning:

Article Title: Protein Synthesis in the Developing Neocortex at Near-Atomic Resolution Reveals Ebp1-Mediated Neuronal Proteostasis at the 60S Tunnel Exit.
Article Snippet: .. Fully Sequenced cDNA (Source BioScience) with primers forward 50- gtctcatcattttggcaaagATGTACCCATACGATGTTCCAGATTACGCTTCGGGCGAAGACGAG-30 and reverse 50-cggccgcgatatcctcgaggTCAGTCCCCAGCTCCATTC-30, followed by cloning into the pCAG-IRES-GFP backbone (Ambrozkiewicz et al., 2018) with the restriction enzyme EcoRI (NEB). ..

Clone Assay:

Article Title: Protein Synthesis in the Developing Neocortex at Near-Atomic Resolution Reveals Ebp1-Mediated Neuronal Proteostasis at the 60S Tunnel Exit
Article Snippet: .. Fully Sequenced cDNA (Source BioScience) with primers forward 5′-gtctcatcattttggcaaagATGTACCCATACGATGTTCCAGATTACGCTTCGGGCGAAGACGAG-3′ and reverse 5′-cggccgcgatatcctcgaggTCAGTCCCCAGCTCCATTC-3′, followed by cloning into the pCAG-IRES-GFP backbone ( Ambrozkiewicz et al., 2018 ) with the restriction enzyme EcoRI (NEB). .. Fully Sequenced cDNA (Source BioScience) with primers forward 5′-gtctcatcattttggcaaagATGTACCCATACGATGTTCCAGATTACGCTTCGGGCGAAGACGAG-3′ and reverse 5′-cggccgcgatatcctcgaggTCAGTCCCCAGCTCCATTC-3′, followed by cloning into the pCAG-IRES-GFP backbone ( Ambrozkiewicz et al., 2018 ) with the restriction enzyme EcoRI (NEB).

other:

Article Title: The architecture of protein synthesis in the developing neocortex at near-atomic resolution reveals Ebp1-mediated neuronal proteostasis at the 60S tunnel exit
Article Snippet: Fully Sequenced cDNA (Source BioScience) with primers forward 5’-gtctcatcattttggcaaagATGTACCCATACGATGTTCCAGATTACGCTTCGGGCGAAGACGAG-3’ and reverse 5’-cggccgcgatatcctcgaggTCAGTCCCCAGCTCCATTC-3’, followed by cloning into the pCAGIG (pCAG-IRES-GFP) backbone ( ) with the restriction enzyme EcoRI (NEB).

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